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Cellular oncogene fos (c-Fos), Sheep Polyclonal Antibody

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Catalog Number

S-045

Product Info

Product Name Cellular oncogene fos (c-Fos), Sheep Polyclonal Antibody
Product Description google Sheep anti-Cellular oncogene fos (c-Fos) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Alternative Names Proto-oncogene protein cFOS; c-FOS
Application(s) IHC-Frozen, WB
Antibody Host Sheep
Antibody Type Polyclonal
Specificity This antiserum shows a high level of specificity for cFOS confirmed by immunohostochemistry. This antiserum is known to react with rat, rabbit and hamster cFOS.
Species Reactivity Hamster, Human, Rabbit, Rat
Immunogen Description A synthetic peptide (MFSGFNADYEASSSRC; aa 2-17) conjugated to diphtheria toxoid has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from cFos protein human, rat, mouse, hamster and cat.
Conjugate Unconjugated
Purity Description Affinity purified
Regulatory Status For research use only.

Specifications

Product Description Sheep anti-Cellular oncogene fos (c-Fos) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Application(s) IHC-Frozen, WB
Application Details IH on PFA fixed, frozen tissues. Not yet tested on formalin fixed, paraffin embedded tissue but expected to react. Affinity purified antibody is extremely powerful. A concentration of 1 -5 µg/mL is recommended most uses with short (1-8 hour) incubations. If using enhanced brightfield or amplified detection methods dilutions and long primary antibody incubations dilutions will need to be increased substantially to inhibit background staining. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.

Not recommended for western blotting applications. Mouse monoclonal antibody M-1752-100 or rabbit polyclonal antibody R-1751-50 are excellent alternatives for western blotting.
Target Cellular oncogene fos (c-Fos)
Specificity This antiserum shows a high level of specificity for cFOS confirmed by immunohostochemistry. This antiserum is known to react with rat, rabbit and hamster cFOS.
Target Host Species Human
Species Reactivity Hamster, Human, Rabbit, Rat
Antibody Host Sheep
Antibody Type Polyclonal
Antibody Isotype IgG
Conjugate Unconjugated
Immunogen Description A synthetic peptide (MFSGFNADYEASSSRC; aa 2-17) conjugated to diphtheria toxoid has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from cFos protein human, rat, mouse, hamster and cat.
Purity Description Affinity purified
Format Lyophilized
Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 50 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage Instructions Store lyophilized product at 2-8°C. After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Batch Number Please see item label.
Expiration Date 12 months after date of receipt (unopened vial).
Alternative Names Proto-oncogene protein cFOS; c-FOS
Uniprot Number P01100
Uniprot Number/Name P01100 (FOS_HUMAN)
Scientific Background FUNCTION: Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. SUBUNIT: Heterodimer. Interacts with DSIPI; this interaction inhibits the binding of active AP1 to its target DNA. Interacts with MAFB. SUBCELLULAR LOCATION: Nucleus. INDUCTION: C-fos expression increases upon a variety of stimuli, including growth factors, cytokines, neurotransmitters, polypeptide hormones, stress and cell injury. SIMILARITY: Belongs to the bZIP family. Fos subfamily. SIMILARITY: Contains 1 bZIP domain
Shipping Temperature 25°C (ambient)
UNSPSC CODE 41116161
Regulatory Status For research use only.

Images, Protocols & SDS

c-Fos was induced in the hypothalamic arcuate nucleus of an adult male Wistar rat with i.p. injection of N-methyl-D-aspartate. The brain was fixed by transcardiac perfusion of the rat with formaldehyde (4%). cFos immunoreactivity was detected on floated cryo-sections of the hypothalamic arcuate nucleus with the S-045-50 primary antibody (1:5000) using the biotinylated secondary antibody-ABC method and nickel-diaminobenzidine chromogen. Photo courtesy of Dr. Erik Hrabovszky, Hungarian Academy of Sciences, Budapest, Hungary.

Citations & References

General References Vanstraaten et al (1983) Proc. Natl. Acad. Sci. 80: 3183
Minson J et al. (1994) Disinhibition of the rostral ventral medulla increases blood pressure and Fos expression in bulbospinal neurons. Brain Res. 1994 May 16;646(1):44-52.