Alternative Namespro-brain nerve growth factor; proNGF; NGF;
Application(s)ELISA, IHC-Frozen, Neutralize, WB
Antibody HostSheep
Antibody TypePolyclonal
SpecificityThe specificity of this antibody has been confirmed by WB. It does NOT crossreact with proBDNF, proNT-3 or mature NGF. Confirmed to react with purified human proNGF and crossreact with mouse and rat proNGF
Species ReactivityHuman, Mouse, Rat
Immunogen DescriptionThe recombinant prodomain fragment of human nerve growth factor
Application DetailsIHC, Immunofluorescence, ELISA, Western Blot, biological neutralization of proNGF. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Targetpro-Nerve growth factor (proNGF)
SpecificityThe specificity of this antibody has been confirmed by WB. It does NOT crossreact with proBDNF, proNT-3 or mature NGF. Confirmed to react with purified human proNGF and crossreact with mouse and rat proNGF
Target Host SpeciesHuman
Species ReactivityHuman, Mouse, Rat
Antibody HostSheep
Antibody TypePolyclonal
Antibody IsotypeMixed
ConjugateUnconjugated
Immunogen DescriptionThe recombinant prodomain fragment of human nerve growth factor
Purity DescriptionWhole serum
FormatLyophilized
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage InstructionsMaintain lyophilized antibody at 2-8°C for up to 12 months after date of receipt. After reconstitution keep undiluted aliquots at -20°C for up to 6 months for higher stability or at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative Namespro-brain nerve growth factor; proNGF; NGF;
Scientific BackgroundNerve growth factor (NGF) is synthesized as a precursor (proNGF) which may be released and have physiological functions to cause cell death. It binds neurotrophin receptor p75 and sortilin and may also be important for the development of nervous system. proNGF is synthesized in target tissues and glia, transported retrogradely and may be released. This antibody is raised in sheep to detect the prodomain of NGF not the mature peptide.
Western blot of proNGF proteins produced in E.Coli. S-080-100 detects the human and mouse form of proNGF as well as the pro-domain peptide. SDS-PAGE: denatured and reduced; Transfer: Tris-Glycine buffer; Membrane: nitrocellulose (0.45 um); Blocking: 5% skim milk in TBST, 2 hours at RT; Primary antibody: overnight at 4°C (1:1000 dilution); Secondary antibody: anti-sheep-HRP (1/3000), 2 hours at RT; Detection: Chemiluminiscence.
Immunofluorescence analysis of proNGF expression in human DU145 prostate cancer cells. Fixed (4% formaldehyde), permeabilized, and blocked (10% normal horse serum, 0.1% Triton X100) DU145 cells were incubated with proNGF antibody S-080-100 (1:1000, red) for 1 hour. Primary antibody binding was visualized with a secondary donkey anti-sheep-PE antibody (1:100, 1 hour incubation). Cell nuclei were stained with Hoechst dye (blue). Magnification: 100x.
The sheep anti-proNGF antibodies recognize recombinant proNGF, the prodomain of NGF and but not mouse mature NGF (50 ng in each lane).
Specific ReferencesSugimoto J et al. (2021). Fabry disease-associated globotriaosylceramide induces mechanical allodynia via activation of signaling through proNGF p75NTR but not mature NGF TrkA. Eur. J. Pharmacol. 895. Application: Neutralising (in-vivo).
Ryu JC et al. (2018). Role of proNGF/p75 signaling in bladder dysfunction after spinal cord injury. J Clin Invest. [Epub ahead of print]. Application: Western Blotting.
General ReferencesSrinivasan B et al. J Biol Chem. 2004 Oct 1;279(40):41839-4 Yune TY et al. J Neurosci. 2007 Jul 18;27(29):7751-61.
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