Sprouty homolog 1 protein (Spry-1), Goat Polyclonal Antibody

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Catalog Number
G-1916

    Product Info

  • Product Name Sprouty homolog 1 protein (Spry-1), Goat Polyclonal Antibody
  • Product Description google Goat anti-Sprouty homolog 1 protein (Spry-1) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, WB, ICC, FC, IHC.
  • Alternative Names SPRY1; hSPRY1; sprouty homolog 1, antagonist of FGF signaling (Drosophila); sprouty (Drosophila) homolog 1 (antagonist of FGF signaling); sprouty, Drosophila, homolog of, 1 (antagonist of FGF signaling)
  • Application(s) FC, ICC, IHC, WB, Pep-ELISA
  • Antibody Host Goat
  • Antibody Type Polyclonal
  • Specificity Reacts with Sprouty from Human, Mouse. NP_005832.1 and NP_955359.1 represent varients of the same protein.
  • Species Reactivity Human, Mouse
  • Immunogen Description A synthetic peptide consisting of amino acids, CPSRGQGKPS
  • Conjugate Unconjugated
  • Purity Description Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Regulatory Status For research use only.

    Specifications

  • Product Description Goat anti-Sprouty homolog 1 protein (Spry-1) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, WB, ICC, FC, IHC.
  • Application(s) FC, ICC, IHC, WB, Pep-ELISA
  • Application Details Peptide ELISA (1:6000), Western Blot (1-3 µg/mL), Flow Cytometry (10 µg/mL), Immunohistochemistry (1-3 µg/mL), Immunofluorescence (10 µg/mL), Flow Cytometry (10 µg/mL). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Sprouty homolog 1 protein (Spry-1)
  • Specificity Reacts with Sprouty from Human, Mouse. NP_005832.1 and NP_955359.1 represent varients of the same protein.
  • Target Host Species Human
  • Species Reactivity Human, Mouse
  • Antibody Host Goat
  • Antibody Type Polyclonal
  • Antibody Isotype IgG
  • Conjugate Unconjugated
  • Immunogen Description A synthetic peptide consisting of amino acids, CPSRGQGKPS
  • Sequence CPSRGQGKPS
  • Purity Description Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Format Liquid antibody. Supplied at 0.5 mg/mL in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
  • Storage Instructions Upon receipt, aliquot and store at -20°C long-term. Store at 2-8°C short-term (up to 2 weeks). Minimize freezing and thawing.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names SPRY1; hSPRY1; sprouty homolog 1, antagonist of FGF signaling (Drosophila); sprouty (Drosophila) homolog 1 (antagonist of FGF signaling); sprouty, Drosophila, homolog of, 1 (antagonist of FGF signaling)
  • Uniprot Number O43609
  • Uniprot Number/Name O43609 (SPY1_HUMAN)
  • Scientific Background Protein sprouty homolog 1 may function as an antagonist of fibroblast growth factor (FGF) pathways and may negatively modulate respiratory organogenesis (Uniprot).
  • Shipping Temperature 2-8°C (on cold packs)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • A. Western Blot: (0.3 µg/mL) staining of Human Kidney lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence. B. Immunofluorescence analysis: of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (4 µg/mL), showing cytoplasmic and Golgi apparatus staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (4 µg/mL).

  • A. Immunofluorescence analysis: paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (4 µg/mL), showing cytoplasmic and Golgi apparatus staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (4 µg/mL). B. Flow cytometric analysis: paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (0.4 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

    Citations & References