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T-lymphocyte activation antigen CD80, Mouse, ELISA assay
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US$547.00
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Catalog Number
BEK-2174
- Product Name T-lymphocyte activation antigen CD80, Mouse, ELISA assay
- Product Description The mouse CD80 Kit is a sandwich ELISA. The capture antibody is a polyclonal mouse CD80 antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known CD80 concentration are added to these wells and allowed to complex with the bound CD80 antibody. A biotinylated mouse CD80 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of CD80 present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse CD80 in samples such as culture supernatant and serum. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Alternative Names T-lymphocyte activation antigen CD80; Activation B7-1 antigen; B7; CD80; Cd80; B7;
- Application(s) ELISA
- Specificity Mouse CD80
- Species Reactivity Mouse
- Immunogen Description The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids D37-K245
- Range 62.5 pg/mL - 4,000 pg/mL
- Sensitivity < 10 pg/mL
- Regulatory Status For research use only.
Product Info
- Product Description The mouse CD80 Kit is a sandwich ELISA. The capture antibody is a polyclonal mouse CD80 antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known CD80 concentration are added to these wells and allowed to complex with the bound CD80 antibody. A biotinylated mouse CD80 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of CD80 present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse CD80 in samples such as culture supernatant and serum. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Application(s) ELISA
- Application Details ELISA. For the quantification of T-lymphocyte activation antigen CD80 in Culture Supernatant, Serum. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
- Target T-lymphocyte activation antigen CD80
- Specificity Mouse CD80
- Target Cross-Reactivity (ELISA) There is no detectable cross-reactivity with other relevant proteins.
- Target Host Species Mouse
- Species Reactivity Mouse
- Immunogen Description The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids D37-K245
- Range 62.5 pg/mL - 4,000 pg/mL
- Sensitivity < 10 pg/mL
- Sample Type Culture Supernatant, Serum
- Detection Method Colorimetric
- Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
- Storage Instructions Store at 2-8°C
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
- Alternative Names T-lymphocyte activation antigen CD80; Activation B7-1 antigen; B7; CD80; Cd80; B7;
- Uniprot Number Q00609
- Uniprot Number/Name Q00609 (CD80_MOUSE)
- Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116158
- Regulatory Status For research use only.
