Parallelism, linearity-of-dilution and spike-and-recovery experiments are essential validation studies that assess the ability of the ELISA assay to measure the true (ie. accurate) amount of antigen in the sample.
Choosing an appropriate non-linear calibration curve regression model is essential for accurate ELISA data analysis, because immunoassays usually do not follow a linear dose-response relationship.
Planning a Flow Cytometry experiment requires knowledge about the protein target of interest, the right antibody and appropriate controls.
Acid-extraction can be used as an alternative tissue extraction method to release BDNF, which can be bound to its receptors and chaperons within many tissues. Samples prepared following this protocol can be assayed for BDNF content by ELISA and also used in Western Blotting to assess BDNF protein expression.
The detection and quantification of mature BDNF by ELISA poses a challenge due to potential cross-reactivity of the proBDNF full-length isoform. This Technical Note demonstrates our efforts in validating mature BDNF quantification by ELISA, and explores the issues relating to using other immunological techniques for validation of BDNF isoform detection by ELISA assay.
In contrast to mature BDNF, less is known about the full-length proBDNF isoform and its physiological role in humans. Specific detection independent of the mature BDNF isoform is one concern, but accurate quantification with fit-for-purpose ELISA assays is equally important. This Technical Note demonstrates how Biosensis has addressed both issues for proBDNF quantification in human serum, and investigates potential pitfalls when using other commercially available proBDNF ELISA assays.
Need a hand in calculating antigen amount in tissue homogenates or cell lysates? Make sense of your ELISA data and get your results publication-ready with this simple Excel spreadsheet-based calculator.