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Testosterone, Mouse, Rat, ELISA assay
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Catalog Number
BEK-2362
Discontinued
- Product Name Testosterone, Mouse, Rat, ELISA assay
- Product Description The Mouse and Rat Testosterone ELISA is a competitive ELISA assay. Quantification is based on the competitive binding between testosterone in the sample and HRP-conjugated testosterone protein to a constant amount of polyclonal anti-testosterone antibody. Standards, controls and samples are incubated with testosterone-HRP conjugate and anti-testosterone detection antibody in anti-rabbit-IgG-coated wells. The coating antibody will capture testosterone-detection antibody complexes. During incubation, a fixed amount of HRP-labeled Testosterone competes with the endogenous Testosterone in the standard, sample, or quality control serum for a fixed number of binding sites of the specific Testosterone antibody. Thus, the amount of Testosterone-HRP conjugate bound to the well progressively decreases as the concentration of Testosterone in sample and standard increases. Unbound Testosterone peroxidase conjugate is then removed and the wells washed. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of testosterone present in the samples. The purpose of this kit is the quantitative determination of mouse and rat testosterone in serum. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures
- Application(s) ELISA
- Specificity Testosterone, competitive ELISA where sample testosterone competes with known standard amounts
- Species Reactivity Mouse, Rat
- Range 0.1 - 18 ng/mL
- Sensitivity 0.1 ng/mL, determined as concentration at blank plus 2x standard deviation of blank (n=20).
- Regulatory Status For research use only.
Product Info
- Product Description The Mouse and Rat Testosterone ELISA is a competitive ELISA assay. Quantification is based on the competitive binding between testosterone in the sample and HRP-conjugated testosterone protein to a constant amount of polyclonal anti-testosterone antibody. Standards, controls and samples are incubated with testosterone-HRP conjugate and anti-testosterone detection antibody in anti-rabbit-IgG-coated wells. The coating antibody will capture testosterone-detection antibody complexes. During incubation, a fixed amount of HRP-labeled Testosterone competes with the endogenous Testosterone in the standard, sample, or quality control serum for a fixed number of binding sites of the specific Testosterone antibody. Thus, the amount of Testosterone-HRP conjugate bound to the well progressively decreases as the concentration of Testosterone in sample and standard increases. Unbound Testosterone peroxidase conjugate is then removed and the wells washed. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of testosterone present in the samples. The purpose of this kit is the quantitative determination of mouse and rat testosterone in serum. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures
- Application(s) ELISA
- Application Details ELISA. For the quantification of Testosterone in Serum. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
- Target Testosterone
- Specificity Testosterone, competitive ELISA where sample testosterone competes with known standard amounts
- Target Cross-Reactivity (ELISA) There is no detectable cross-reactivity with other relevant proteins.
- Target Host Species Mouse
- Species Reactivity Mouse, Rat
- Range 0.1 - 18 ng/mL
- Sensitivity 0.1 ng/mL, determined as concentration at blank plus 2x standard deviation of blank (n=20).
- Sample Type Serum
- Detection Method Colorimetric
- Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), ready-to-use testosterone standards, testosterone-HRP conjugate, detection antibody, buffer solutions, TMB substrate, stop solution and detailed protocols.
- Storage Instructions Store at 2-8°C
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date This kit may be stored for up to 12 months at 2°C to 8°C from the date of shipment.
- Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116158
- Regulatory Status For research use only.
