Transcription factor SOX-2 (SOX2), Goat Polyclonal Antibody

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Catalog Number
G-1919

    Product Info

  • Product Name Transcription factor SOX-2 (SOX2), Goat Polyclonal Antibody
  • Product Description google Goat anti-Transcription factor SOX-2 (SOX2) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, WB, ICC, FC.
  • Alternative Names SOX2; SRY (sex determining region Y)-box 2; ANOP3; MGC2413 ; SRY-related HMG-box gene 2; sex-determining region Y-box 2; transcription factor SOX2
  • Application(s) FC, ICC, WB, Pep-ELISA
  • Antibody Host Goat
  • Antibody Type Polyclonal
  • Specificity Reacts with SOX2 from Human.
  • Species Reactivity Bovine, Dog, Human, Mouse, Rat
  • Immunogen Description A synthetic peptide consisting of amino acids, YNMMETELKPPGPQQ-C
  • Conjugate Unconjugated
  • Purity Description Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Regulatory Status For research use only.

    Specifications

  • Product Description Goat anti-Transcription factor SOX-2 (SOX2) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, WB, ICC, FC.
  • Application(s) FC, ICC, WB, Pep-ELISA
  • Application Details Peptide ELISA (1:32,000), Western Blot (0.1-0.3 µg/mL), Immunocytochemistry (10 µg/mL),Flow Cytometry (10 µg/mL). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Transcription factor SOX-2 (SOX2)
  • Specificity Reacts with SOX2 from Human.
  • Target Host Species Human
  • Species Reactivity Bovine, Dog, Human, Mouse, Rat
  • Antibody Host Goat
  • Antibody Type Polyclonal
  • Antibody Isotype IgG
  • Conjugate Unconjugated
  • Immunogen Description A synthetic peptide consisting of amino acids, YNMMETELKPPGPQQ-C
  • Sequence YNMMETELKPPGPQQ
  • Purity Description Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Format Liquid antibody. Supplied at 0.5 mg/mL in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
  • Storage Instructions Upon receipt, aliquot and store at -20°C long-term. Store at 2-8°C short-term (up to 2 weeks). Minimize freezing and thawing.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names SOX2; SRY (sex determining region Y)-box 2; ANOP3; MGC2413 ; SRY-related HMG-box gene 2; sex-determining region Y-box 2; transcription factor SOX2
  • Uniprot Number P48431
  • Uniprot Number/Name P48431 (SOX2_HUMAN)
  • Scientific Background Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency. May function as a switch in neuronal development. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal (By similarity). Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation
  • Shipping Temperature 2-8°C (on cold packs)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • A: Western Blot: G-1919-100 (0.3µg/ml) staining of MCF7 (A) HEK293 (B) and MCF7 nuclear (C) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
    B: Flow cytometry: G-1919-100 analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary (G-1919-100) incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

    Citations & References