Product DescriptionGoat anti-Transient receptor potential cation channel subfamily M member 2 (TRPM2) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, ICC, FC, IHC.
Application(s)FC, ICC, IHC, Pep-ELISA
Application DetailsPeptide ELISA (1:6,000), Immunohistochemistry (3.75 µg/mL), Immunocytochemistry (10 µg/mL), Flow Cytometry (10 µg/mL). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetTransient receptor potential cation channel subfamily M member 2 (TRPM2)
SpecificityReacts with TRPM2 from Human.
Target Host SpeciesHuman
Species ReactivityBovine, Human, Mouse
Immunogen DescriptionA synthetic peptide consisting of amino acids, C-QQKQRPEQKIED
Purity DescriptionPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
FormatLiquid antibody. Supplied at 0.5 mg/mL in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Storage InstructionsUpon receipt, aliquot and store at -20°C long-term. Store at 2-8°C short-term (up to 2 weeks). Minimize freezing and thawing.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesTRPM2; transient receptor potential cation channel, subfamily M, member 2; EREG1; KNP3; LTRPC2; MGC133383; NUDT9H; NUDT9L1; TRPC7; estrogen responsive element associated gene 1; long transient receptor potential channel 2; transient receptor potential channel 7; Transient receptor potential melastatin 2;
Scientific BackgroundPlays a role in mediating behavorial and physiological responses to moderate heat and thereby contributes to body temperature homeostasis. Plays a role in insulin secretion, a process that requires increased cytoplasmic Ca2+ levels
A. Immunofluorescenceanalysis: of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nuclear membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL). B. Flow cytometryanalysis: of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control:Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
A. Human Pancreas Staining: (3.75 µg/mL) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining. B. Human Cortex Staining: (3.75 µg/mL) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.