Immunogen DescriptionA synthetic peptide consisting of amino acids, C-RDNSGRRGGSRLPE
ConjugateUnconjugated
Purity DescriptionPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Product DescriptionGoat anti-Translocator protein Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, WB, IHC, FC.
Application(s)FC, IHC, WB, Pep-ELISA
Application DetailsPeptide ELISA (1:16000), Western Blot (0.1-0.3 µg/mL). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetTranslocator protein
SpecificityReacts with PBR (mouse) from Mouse.
Target Host SpeciesMouse
Species ReactivityHuman, Mouse, Rat
Antibody HostGoat
Antibody TypePolyclonal
Antibody IsotypeIgG
ConjugateUnconjugated
Immunogen DescriptionA synthetic peptide consisting of amino acids, C-RDNSGRRGGSRLPE
SequenceRDNSGRRGGSRLPE
Purity DescriptionPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
FormatLiquid antibody. Supplied at 0.5 mg/mL in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Storage InstructionsUpon receipt, aliquot and store at -20°C long-term. Store at 2-8°C short-term (up to 2 weeks). Minimize freezing and thawing.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundCan bind protoporphyrin IX and may play a role in the transport of porphyrins and heme (By similarity). Was initially identified as peripheral-type benzodiazepine receptor; can also bind isoquinoline carboxamides. Promotes the transport of cholesterol across mitochondrial membranes and may play a role in lipid metabolism (PubMed:9832438, PubMed:24814875), but its precise physiological role is controversial. According to some reports, it is not required for steroid hormone biosynthesis (PubMed:24174323, PubMed:24936060).
A. Western Blot: (1 µg/mL) staining of Mouse Spleen lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence. B. Flow cytometryanalysis: of paraformaldehyde fixed NIH3T3 cells (blue line), permeabilized with 0.5%Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
A. Immunofluorescence analysis: of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing mitochondrial /cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL). B. Immunofluorescence analysis: of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
A. Human Renal Tubule Staining: (3.75 µg/mL) staining of paraffin embedded Human Renal Tubules. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. B. Human Squamous Epithelium Staining: (3.75 µg/mL) staining of paraffin embedded Human Squamous Epithelium. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.
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