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Triggering receptor expressed on myeloid cells 2 (TREM2), Human, ELISA assay
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Catalog Number
BEK-2339
Discontinued
- Product Name Triggering receptor expressed on myeloid cells 2 (TREM2), Human, ELISA assay
- Product Description The human TREM2 kit is a sandwich ELISA. The capture antibody is a mouse monoclonal TREM2 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known TREM2 concentration are added to these wells and allowed to complex with the bound TREM2 antibody. A biotinylated goat polyclonal TREM2 antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of TREM2 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human TREM2 in samples such as culture supernatant, serum, plasma (EDTA) and plasma (Heparin). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Alternative Names Triggering receptor expressed on myeloid cells 2; TREM-2; Triggering receptor expressed on monocytes 2; TREM2
- Application(s) ELISA
- Specificity Human
- Species Reactivity Human
- Immunogen Description The standard and immunogen for this kit is recombinant TREM2 protein produced in and purified from an E.coli expression system using immunogen sequence H19-E167.
- Range 31.2 - 2,000 pg/mL
- Sensitivity < 10 pg/mL
- Regulatory Status For research use only.
Product Info
- Product Description The human TREM2 kit is a sandwich ELISA. The capture antibody is a mouse monoclonal TREM2 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known TREM2 concentration are added to these wells and allowed to complex with the bound TREM2 antibody. A biotinylated goat polyclonal TREM2 antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of TREM2 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human TREM2 in samples such as culture supernatant, serum, plasma (EDTA) and plasma (Heparin). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Application(s) ELISA
- Application Details ELISA. For the quantification of Triggering receptor expressed on myeloid cells 2 (TREM2) in Culture Supernatant, Serum, Plasma (Heparin), Plasma (EDTA). Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
- Target Triggering receptor expressed on myeloid cells 2 (TREM2)
- Specificity Human
- Target Cross-Reactivity (ELISA) Does not cross-react with other relevant proteins
- Target Host Species Human
- Species Reactivity Human
- Immunogen Description The standard and immunogen for this kit is recombinant TREM2 protein produced in and purified from an E.coli expression system using immunogen sequence H19-E167.
- Range 31.2 - 2,000 pg/mL
- Sensitivity < 10 pg/mL
- Sample Type Culture Supernatant, Plasma (EDTA), Plasma (Heparin), Serum
- Detection Method Colorimetric
- Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
- Storage Instructions Store at 2-8°C
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date 6 months after date of receipt if unopened
- Alternative Names Triggering receptor expressed on myeloid cells 2; TREM-2; Triggering receptor expressed on monocytes 2; TREM2
- Uniprot Number Q9NZC2
- Uniprot Number/Name Q9NZC2 (TREM2_HUMAN)
- Scientific Background The protein Triggering receptor expressed on myeloid cells 2 (TREM2) forms a receptor signaling complex with the TYROprotein tyrosine kinase binding protein (TYROBP). TREM2 has roles in the immune response and may be involved in chronic inflammation by triggering the production of constitutive inflammatory cytokines. Defects in this gene are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL). Alternative splicing results in multiple transcript variants encoding isoforms with differing subcellular locations.
- Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116158
- Regulatory Status For research use only.
