1800 605-5127
+61 (0)8 8352 7711
Tumor necrosis factor alpha (TNF-alpha), Mouse, ELISA assay
As low as
US$577.00
Only %1 left
Catalog Number
BEK-2102
- Product Name Tumor necrosis factor alpha (TNF-alpha), Mouse, ELISA assay
- Product Description The mouse TNF-alpha Kit is a sandwich ELISA. The capture antibody is an anti-mouse TNF-alpha antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known TNF-alpha concentration are added to these wells and allowed to complex with the bound TNF-alpha antibody. A biotinylated mouse TNF-alpha antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of TNF-alpha present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse TNF-alpha in samples such as culture supernatant, serum, cell lysates, plasma (Heparin) and plasma (EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Alternative Names TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a; Cachectin; TNF; TNFA; TNFSF2;
- Application(s) ELISA
- Specificity Mouse TNF-alpha
- Species Reactivity Mouse
- Immunogen Description The standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids L80-L235
- Range 15.6 pg/mL - 1,000 pg/mL (cell culture supernates), 7.8 pg/mL - 500 pg/mL (mouse serum, plasma)
- Sensitivity < 1 pg/mL
- Regulatory Status For research use only.
Product Info
- Product Description The mouse TNF-alpha Kit is a sandwich ELISA. The capture antibody is an anti-mouse TNF-alpha antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known TNF-alpha concentration are added to these wells and allowed to complex with the bound TNF-alpha antibody. A biotinylated mouse TNF-alpha antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of TNF-alpha present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse TNF-alpha in samples such as culture supernatant, serum, cell lysates, plasma (Heparin) and plasma (EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Application(s) ELISA
- Application Details ELISA. For the quantification of Tumor necrosis factor alpha (TNF-alpha) in Culture Supernatant, Serum, Cell Lysates, Plasma (Heparin), Plasma (EDTA). Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
- Target Tumor necrosis factor alpha (TNF-alpha)
- Specificity Mouse TNF-alpha
- Target Cross-Reactivity (ELISA) There is no detectable cross-reactivity with other relevant proteins.
- Target Host Species Mouse
- Species Reactivity Mouse
- Immunogen Description The standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids L80-L235
- Range 15.6 pg/mL - 1,000 pg/mL (cell culture supernates), 7.8 pg/mL - 500 pg/mL (mouse serum, plasma)
- Sensitivity < 1 pg/mL
- Sample Type Cell Lysates, Culture Supernatant, Plasma (EDTA), Plasma (Heparin), Serum
- Detection Method Colorimetric
- Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
- Storage Instructions Store at 2-8°C
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
- Alternative Names TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a; Cachectin; TNF; TNFA; TNFSF2;
- Uniprot Number P06804
- Uniprot Number/Name P06804 (TNFA_MOUSE)
- Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116158
- Regulatory Status For research use only.
Specifications
-
Specific References
Cavanagh C, Tse YC, Nguyen HB, Krantic S, Breitner JCS, Quirion R, Wong TP (2016) Inhibiting Tumor Necrosis Factor-_ before amyloidosis prevents synaptic deficits in an Alzheimer's disease model. Neurobiol Aging. 2016;47:41-9
Domene A,Cavanagh C, Page G, Bodard S, Klein C, Delarasse C, Chalon S, Krantic S (2016) Expression of Phenotypic Astrocyte Marker Is Increased in a Transgenic Mouse Model of Alzheimer's Disease versus Age-Matched Controls: A Presymptomatic Stage Study. Int J Alzheimers Dis. 2016;2016:5696241
Kunos, G, Jourdan T, Michael P, Aouadi M (2016) Glucan-Encapsulated siRNA for Treating Type 2 Diabetes Mellitus. US Patent Application 20160138028
Jourdan T, Godlewski G, Cinar R, Bertola A, Szanda G, Liu J, Tam J, Han T, Mukhopadhyay B, Skarulis MC, Ju C, Aouadi M, Czech MP, Kunos G. (2013) Activation of the Nlrp3 inflammasome in infiltrating macrophages by endocannabinoids mediates beta cell loss in type 2 diabetes. Nat. Med Sep;19(9):1132-40 2013 [E print]
Cavanagh C et al (2013) _CTF-Correlated Burst of Hippocampal TNF-alpha Occurs at a Very Early, Pre-Plaque Stage in the TgCRND8 Mouse Model of Alzheimer's Disease. J Alzheimers Dis. 2013 Apr 11.
Vitner EB et al (2012) Contribution of brain inflammation to neuronal cell death in neuronopathic forms of Gaucher's disease. Brain. 2012 Jun;135(Pt 6):1724-35.