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Tumor necrosis factor alpha (TNF-alpha), Rat, ELISA assay
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Catalog Number
BEK-2101
- Product Name Tumor necrosis factor alpha (TNF-alpha), Rat, ELISA assay
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Product Description
The rat TNF-alpha Kit is a sandwich ELISA. The capture antibody is a monoclonal rat TNF-alpha antibody pre-coated onto the 96-well strip plates provided in the kit. Rat test samples and standards of known TNF-alpha concentration are added to these wells and allowed to complex with the bound TNF-alpha antibody. A biotinylated rat TNF-alpha polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of TNF-alpha present in the samples. The purpose of this kit is the in-vitro quantitative determination of rat TNF-alpha in samples such as culture supernatant, serum, cell lysates, plasma (Heparin) and plasma (EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Alternative Names TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a; Cachectin; TNF; TNFA; TNFSF2;
- Application(s) ELISA
- Specificity Rat TNF-alpha
- Species Reactivity Rat
- Immunogen Description The standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids L80-L235
- Range 15.6 pg/mL - 1,000 pg/mL (cell culture supernates), 7.8 pg/mL - 500 pg/mL (serum, plasma)
- Sensitivity < 1 pg/mL
- Regulatory Status For research use only.
Product Info
-
Product Description
The rat TNF-alpha Kit is a sandwich ELISA. The capture antibody is a monoclonal rat TNF-alpha antibody pre-coated onto the 96-well strip plates provided in the kit. Rat test samples and standards of known TNF-alpha concentration are added to these wells and allowed to complex with the bound TNF-alpha antibody. A biotinylated rat TNF-alpha polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of TNF-alpha present in the samples. The purpose of this kit is the in-vitro quantitative determination of rat TNF-alpha in samples such as culture supernatant, serum, cell lysates, plasma (Heparin) and plasma (EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Application(s) ELISA
- Application Details ELISA. For the quantification of Tumor necrosis factor alpha (TNF-alpha) in Culture Supernatant, Serum, Cell Lysates, Plasma (Heparin), Plasma (EDTA). Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
- Target Tumor necrosis factor alpha (TNF-alpha)
- Specificity Rat TNF-alpha
- Target Cross-Reactivity (ELISA) Human sVEGFR1 isoforms
- Target Host Species Rat
- Species Reactivity Rat
- Immunogen Description The standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids L80-L235
- Range 15.6 pg/mL - 1,000 pg/mL (cell culture supernates), 7.8 pg/mL - 500 pg/mL (serum, plasma)
- Sensitivity < 1 pg/mL
- Sample Type Cell Lysates, Culture Supernatant, Plasma (EDTA), Plasma (Heparin), Serum
- Detection Method Colorimetric
- Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
- Storage Instructions Store at 2-8°C
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
- Alternative Names TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a; Cachectin; TNF; TNFA; TNFSF2;
- Uniprot Number P16599
- Uniprot Number/Name P16599 (TNFA_RAT)
- Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116158
- Regulatory Status For research use only.
Specifications
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Specific References
Zhang Yet al. (2021) SIRT1 and SIRT2 modulators reduce LPS-induced inflammation in HAPI microglial cells and protect SH-SY5Y neuronal cells in vitro. J Neural Transm (Vienna). 128(5):631-644 Application: Human, culture supernatant.
Du H et al. (2021) Small interfering RNA-induced silencing lncRNA PVT1 inhibits atherosclerosis via inactivating the MAPK/NF-κB pathway. Aging (Albany NY). 13(21):24449-24463 Application: Serum.