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Tumor necrosis factor ligand superfamily member 8 (CD30), Mouse, ELISA assay
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US$547.00
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Catalog Number
BEK-2164
- Product Name Tumor necrosis factor ligand superfamily member 8 (CD30), Mouse, ELISA assay
- Product Description The mouse CD30 ligand (CD30L) Kit is a sandwich ELISA. The capture antibody is a monoclonal mouse CD30L antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known CD30L concentration are added to these wells and allowed to complex with the bound CD30L antibody. A biotinylated mouse CD30L polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of CD30L present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse CD30L in samples such as culture supernatant and serum. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Alternative Names Tumor necrosis factor ligand superfamily member 8; CD153; Tnfsf8; CD30 ligand;
- Application(s) ELISA
- Specificity Mouse CD30L
- Species Reactivity Mouse
- Immunogen Description The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids Q68-D239
- Range 15.6 pg/mL - 1,000 pg/mL
- Sensitivity < 6 pg/mL
- Regulatory Status For research use only.
Product Info
- Product Description The mouse CD30 ligand (CD30L) Kit is a sandwich ELISA. The capture antibody is a monoclonal mouse CD30L antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known CD30L concentration are added to these wells and allowed to complex with the bound CD30L antibody. A biotinylated mouse CD30L polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of CD30L present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse CD30L in samples such as culture supernatant and serum. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Application(s) ELISA
- Application Details ELISA. For the quantification of Tumor necrosis factor ligand superfamily member 8 (CD30) in Culture Supernatant, Serum. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
- Target Tumor necrosis factor ligand superfamily member 8 (CD30)
- Specificity Mouse CD30L
- Target Host Species Mouse
- Species Reactivity Mouse
- Immunogen Description The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids Q68-D239
- Range 15.6 pg/mL - 1,000 pg/mL
- Sensitivity < 6 pg/mL
- Sample Type Culture Supernatant, Serum
- Detection Method Colorimetric
- Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
- Storage Instructions Store at 2-8°C
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
- Alternative Names Tumor necrosis factor ligand superfamily member 8; CD153; Tnfsf8; CD30 ligand;
- Uniprot Number P32972
- Uniprot Number/Name P32972 (TNFL8_MOUSE
- Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116158
- Regulatory Status For research use only.
