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Tumor necrosis factor receptor superfamily member 5 (CD40), Mouse, ELISA assay
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US$547.00
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Catalog Number
BEK-2193
- Product Name Tumor necrosis factor receptor superfamily member 5 (CD40), Mouse, ELISA assay
- Product Description The mouse CD40 Kit is a sandwich ELISA. The capture antibody is a polyclonal mouse CD40 antibody pre-coated onto the 96-well strip plates provided in the kit. Test samples and standards of known CD40 concentration are added to these wells and allowed to complex with the bound CD40 antibody. A biotinylated mouse polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of CD40 present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse CD40 in samples such as culture supernatant, cell lysates, serum, plasma (Heparin) and plasma (EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Alternative Names Tumor necrosis factor receptor superfamily member 5; B-cell surface antigen CD40; Bp50; CD40L receptor; CDw40; CD40; TNFRSF5;
- Application(s) ELISA
- Specificity Mouse CD40
- Species Reactivity Mouse
- Immunogen Description The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids V24-R193
- Range 15.6 pg/mL - 1,000 pg/mL
- Sensitivity < 1pg/mL
- Regulatory Status For research use only.
Product Info
- Product Description The mouse CD40 Kit is a sandwich ELISA. The capture antibody is a polyclonal mouse CD40 antibody pre-coated onto the 96-well strip plates provided in the kit. Test samples and standards of known CD40 concentration are added to these wells and allowed to complex with the bound CD40 antibody. A biotinylated mouse polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of CD40 present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse CD40 in samples such as culture supernatant, cell lysates, serum, plasma (Heparin) and plasma (EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Application(s) ELISA
- Application Details ELISA. For the quantification of Tumor necrosis factor receptor superfamily member 5 (CD40) in Culture Supernatant, Cell Lysates, Serum, Plasma (EDTA), Plasma (Heparin). Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
- Target Tumor necrosis factor receptor superfamily member 5 (CD40)
- Specificity Mouse CD40
- Target Host Species Mouse
- Species Reactivity Mouse
- Immunogen Description The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids V24-R193
- Range 15.6 pg/mL - 1,000 pg/mL
- Sensitivity < 1pg/mL
- Sample Type Cell Lysates, Culture Supernatant, Plasma (EDTA), Plasma (Heparin), Serum
- Detection Method Colorimetric
- Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
- Storage Instructions Store at 2-8°C
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
- Alternative Names Tumor necrosis factor receptor superfamily member 5; B-cell surface antigen CD40; Bp50; CD40L receptor; CDw40; CD40; TNFRSF5;
- Uniprot Number P27512
- Uniprot Number/Name P27512 (TNR5_MOUSE)
- Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116158
- Regulatory Status For research use only.