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Tumor Necrosis Factor receptor type II, soluble, Human, ELISA assay
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Catalog Number
BEK-2103
- Product Name Tumor Necrosis Factor receptor type II, soluble, Human, ELISA assay
- Product Description The human Soluble Tumor Necrosis Factor receptor type II (TNF-R2) Kit is a sandwich ELISA. The capture antibody is a polyclonal human TNF-R2 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known TNF-R2 concentration are added to these wells and allowed to complex with the bound TNF-R2 antibody. A biotinylated human TNF-R2 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of TNF-R2 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human TNF-R2 in samples such as culture supernatant, serum, plasma (Citrate), plasma (EDTA), plasma (Heparin) and urine. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Alternative Names Tumor necrosis factor receptor superfamily member 1B; Tumor necrosis factor receptor 2; TNF-R2; Tumor necrosis factor receptor type II; TNF-RII; TNFR-II; p75; p80 TNF-alpha receptor; CD120b; Etanercept;
- Application(s) ELISA
- Specificity Human Soluble Tumor Necrosis Factor receptor type II
- Species Reactivity Human
- Immunogen Description The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids L23-D257
- Range 7.8 pg/mL - 500 pg/mL
- Sensitivity < 2 pg/mL
- Regulatory Status For research use only.
Product Info
- Product Description The human Soluble Tumor Necrosis Factor receptor type II (TNF-R2) Kit is a sandwich ELISA. The capture antibody is a polyclonal human TNF-R2 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known TNF-R2 concentration are added to these wells and allowed to complex with the bound TNF-R2 antibody. A biotinylated human TNF-R2 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of TNF-R2 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human TNF-R2 in samples such as culture supernatant, serum, plasma (Citrate), plasma (EDTA), plasma (Heparin) and urine. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Application(s) ELISA
- Application Details ELISA. For the quantification of Tumor Necrosis Factor receptor type II, soluble in Culture Supernatant, Serum, Plasma (Heparin), Plasma (EDTA), Plasma (Citrate), Urine. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
- Target Tumor Necrosis Factor receptor type II, soluble
- Specificity Human Soluble Tumor Necrosis Factor receptor type II
- Target Cross-Reactivity (ELISA) There is no detectable cross-reactivity with other relevant proteins.
- Target Host Species Human
- Species Reactivity Human
- Immunogen Description The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids L23-D257
- Range 7.8 pg/mL - 500 pg/mL
- Sensitivity < 2 pg/mL
- Sample Type Culture Supernatant, Plasma (Citrate), Plasma (EDTA), Plasma (Heparin), Serum, Urine
- Detection Method Colorimetric
- Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
- Storage Instructions Store at 2-8°C
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
- Alternative Names Tumor necrosis factor receptor superfamily member 1B; Tumor necrosis factor receptor 2; TNF-R2; Tumor necrosis factor receptor type II; TNF-RII; TNFR-II; p75; p80 TNF-alpha receptor; CD120b; Etanercept;
- Uniprot Number P20333
- Uniprot Number/Name P20333 (TNR1B_HUMAN)
- Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116158
- Regulatory Status For research use only.
