Product DescriptionTyrosine Kinase Receptor A (TrkA)-Fc Chimera, Purified Recombinant Protein, suitable to Block.
TargetTyrosine Kinase Receptor A (TrkA)
Target Host SpeciesHuman
Antigen Produced InHEK293
SequenceA DNA sequence encoding the signal peptide and extracellular domain of human TrkA (amino acids 1- 413) was fused to the Fc region of human IgG1 (amino acids 104-330). The chimeric protein was expressed in modified human 293 cells.
Purity Description>95%, as determined by SDS-PAGE.
Purity %> 95%
FormatProtein G purified.
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute with sterile PBS, pH 7.4 to give a concentration of 0.5 mg/mL.
Storage InstructionsLyophilized product should be stored at 2 to 8°C. Following reconstitution, short-term storage at 2-8°C is recommended and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesTropomyosin-related kinase receptor; High affinity nerve growth factor receptor; Neurotrophic tyrosine kinase receptor type 1; TRK1 transforming tyrosine kinase protein; p140-TrkA; Trk-A; NTRK1; TRK
Scientific BackgroundTrkA is a member of the neurotrophic tyrosine kinase receptor family. It is a membrane-bound receptor that upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. TrkA is required for high-affinity binding to nerve growth factor (NGF), neurotrophin-3 and neurotrophin-4/5 but not brain-derived neurotrophic factor (BDNF). TrkA leads to cell differentiations and may play a role in specifying sensory neuron subtypes. It has a crucial role in the development and function of the nociceptive reception system as well as establishment of thermal regulation via sweating. SUBUNIT: Exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures. SUBCELLULAR LOCATION: TrkA is a heavily glycosylated transmembrane protein and contains 13 potential N-linked glycosylation sites. The TrkA-Fc chimera has N-linked and may have O-linked oligosaccharides.