Product DescriptionTyrosine Kinase Receptor B (TrkB)-Fc Chimera, Human, Purified Recombinant Protein, suitable for IP and to Block.
Application DetailsThe TrkB-Fc chimera bound to protein A sepharose beads is able to pull down its ligand BDNF. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetTyrosine Kinase Receptor B (TrkB)
Target Host SpeciesHuman
Antigen Produced InHEK293
SequenceA DNA sequence encoding the signal peptide and extracellular domain of human TrkB (amino acids 1- 428) was fused to the Fc region of human IgG1 (amino acids 93-330). The chimeric protein was expressed in modified human 293 cells.
Purity Description>95%, as determined by SDS-PAGE and visualized by silver stain.
Purity %> 95%
FormatThe chimera consists of 20-40% carbohydrate by weight.
Reconstitution InstructionsReconstitute with sterile-filtered phosphate-buffered saline to a concentration of 20 µg/mL.
Storage InstructionsLyophilized products should be stored at 2 to 8°C. Following reconstitution, short-term storage at 2-8°C is recommended and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundTyrosine Kinase Receptor B (TrkB) is a high affinity receptor for brain-derived neurotrophic factor (BDNF) and neurotrophin-4/5. It also binds neurotrophin 3 (NT-3) with lower affinity. TrkB does not bind nerve growth factor (NGF). TrkB is involved in the development and/or maintenance of the nervous system and is widely expressed, predominantly in nervous tissue. TrkB is a heavily glycosylated type I transmembrane protein. The TrkB-Fc chimera contains 13 potential N-linked glycosylation sites.
Specific ReferencesBeros J et al. (2021) Age Related Response of Neonatal Rat Retinal Ganglion Cells to Reduced TrkB Signaling in vitro and in vivo. Front Cell Dev Biol. 9:671087.Application: Rat, Block.
Beros J (2020) Pretreatment of ovaries with collagenase before vitrification keeps the ovarian reserve by maintaining cell-cell adhesion integrity in ovarian follicles. PhD Thesis.Application: Rat, Block.