1800 605-5127
+61 (0)8 8352 7711
Tyrosine Kinase Receptor B (TrkB), Mouse, ELISA assay
As low as
US$547.00
Only %1 left
Catalog Number
BEK-2178
- Product Name Tyrosine Kinase Receptor B (TrkB), Mouse, ELISA assay
- Product Description The mouse TrkB Kit is a sandwich ELISA. The capture antibody is a monoclonal rat TrkB antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known TrkB concentration are added to these wells and allowed to complex with the bound TrkB antibody. A biotinylated goat TrkB polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of TrkB present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse TrkB in samples such as culture supernatant, cell lysates, serum, plasma (Heparin) and plasma (EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Alternative Names Tropomyosin-related kinase receptor; BDNF/NT-3 growth factors receptor; Neurotrophic tyrosine kinase receptor type 2; TrkB tyrosine kinase; GP145-TrkB; Trk-B; TrkB; NTRK2; TRKB
- Application(s) ELISA
- Specificity Mouse. Cross-reacts with human and rat TrkB.
- Species Reactivity Mouse
- Immunogen Description The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids C32-H429
- Range 93.8 pg/mL - 6,000 pg/mL
- Sensitivity < 5 pg/mL
- Regulatory Status For research use only.
Product Info
- Product Description The mouse TrkB Kit is a sandwich ELISA. The capture antibody is a monoclonal rat TrkB antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known TrkB concentration are added to these wells and allowed to complex with the bound TrkB antibody. A biotinylated goat TrkB polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of TrkB present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse TrkB in samples such as culture supernatant, cell lysates, serum, plasma (Heparin) and plasma (EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Application(s) ELISA
- Application Details ELISA. For the quantification of Tyrosine Kinase Receptor B (TrkB) in Culture Supernatant, Cell Lysates, Serum, Plasma (EDTA), Plasma (Heparin). Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
- Target Tyrosine Kinase Receptor B (TrkB)
- Specificity Mouse. Cross-reacts with human and rat TrkB.
- Target Host Species Mouse
- Species Reactivity Mouse
- Immunogen Description The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids C32-H429
- Range 93.8 pg/mL - 6,000 pg/mL
- Sensitivity < 5 pg/mL
- Sample Type Cell Lysates, Culture Supernatant, Plasma (EDTA), Plasma (Heparin), Serum
- Detection Method Colorimetric
- Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
- Storage Instructions Store at 2-8°C
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
- Alternative Names Tropomyosin-related kinase receptor; BDNF/NT-3 growth factors receptor; Neurotrophic tyrosine kinase receptor type 2; TrkB tyrosine kinase; GP145-TrkB; Trk-B; TrkB; NTRK2; TRKB
- Uniprot Number P15209
- Uniprot Number/Name P15209 (NTRK2_MOUSE)
- Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116158
- Regulatory Status For research use only.
Specifications
-
Specific References
Maejima H, Ninuma S, Okuda A, Inoue T, Hayashi M (2018) Exercise and low-level GABAA receptor inhibition modulate locomotor activity and the expression of BDNF accompanied by changes in epigenetic regulation in the hippocampus. Neurosci Lett. [Epub ahead of print]. Application: Mouse hippocampus homogenates.
Maejima H, Kanemura N, Kokubun T, Murata K, Takayanagi K (2017) Exercise enhances cognitive function and neurotrophin expression in the hippocampus accompanied by changes in epigenetic programming in senescence-accelerated mice. Neurosci Lett. doi: 10.1016/j.neulet.2017.11.023. [Epub ahead of print]. Application: Mouse hippocampus homogenates.