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Tyrosine Kinase Receptor C (TrkC), Human, Mouse, Rat, ELISA assay
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US$757.00
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Catalog Number
BEK-2357
- Product Name Tyrosine Kinase Receptor C (TrkC), Human, Mouse, Rat, ELISA assay
- Product Description The Mouse TrkC ELISA kit is a sandwich ELISA. The capture antibody is a rat monoclonal TrkC antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known TrkC concentration are added to these wells and allowed to complex with the bound TrkC antibody. A biotinylated TrkC goat polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of TrkC present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse TrkC in samples such as culture supernatant and cell lysates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Alternative Names Ntrk3; NT-3 growth factor receptor; GP145-TrkC; Trk-C; Neurotrophic tyrosine kinase receptor type 3; TrkC tyrosine kinase; Trk-C;
- Application(s) ELISA
- Specificity Mouse TrkC
- Species Reactivity Human, Mouse, Rat
- Immunogen Description The standard for this kit is recombinant mouse TrkC produced in NS0 cells; Immunogen sequence: C32-T429
- Range 156 pg/mL - 10,000 pg/mL
- Sensitivity < 10 pg/mL
- Regulatory Status For research use only.
Product Info
- Product Description The Mouse TrkC ELISA kit is a sandwich ELISA. The capture antibody is a rat monoclonal TrkC antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known TrkC concentration are added to these wells and allowed to complex with the bound TrkC antibody. A biotinylated TrkC goat polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of TrkC present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse TrkC in samples such as culture supernatant and cell lysates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Application(s) ELISA
- Application Details ELISA. For the quantification of Tyrosine Kinase Receptor C (TrkC) in Culture Supernatant, Cell Lysates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
- Target Tyrosine Kinase Receptor C (TrkC)
- Specificity Mouse TrkC
- Target Cross-Reactivity (ELISA) There is no detectable cross-reactivity with other relevant proteins.
- Target Host Species Mouse
- Species Reactivity Human, Mouse, Rat
- Immunogen Description The standard for this kit is recombinant mouse TrkC produced in NS0 cells; Immunogen sequence: C32-T429
- Range 156 pg/mL - 10,000 pg/mL
- Sensitivity < 10 pg/mL
- Sample Type Cell Lysates, Culture Supernatant
- Detection Method Colorimetric
- Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), mouse TrkC protein standards, mouse TrkC biotinylated antibody, Avidin-Biotin-Peroxidase Complex, sample, antibody and avidin-biotin peroxidase diluents, substrate reagent, stop solution, wash buffer, plate sealers and detailed protocols.
- Storage Instructions Store at 2-8°C
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date 6 months after date of receipt if unopened
- Alternative Names Ntrk3; NT-3 growth factor receptor; GP145-TrkC; Trk-C; Neurotrophic tyrosine kinase receptor type 3; TrkC tyrosine kinase; Trk-C;
- Uniprot Number Q6VNS1
- Uniprot Number/Name Q6VNS1 (NTRK3_MOUSE)
- Scientific Background TrkC is a member of the neurotrophic tyrosine receptor kinase family. TrkC is a membrane-bound receptor that upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. TrkC is the receptor for neurotrophin-3 (NT-3).Signalling through TrkC leads to cell differentiation and may play a role in the development of proprioceptive neurons that sense body position. SUBUNIT: Exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures. SUBCELLULAR LOCATION: Membrane; single-pass type I membrane protein. ALTERNATIVE PRODUCTS: 4 named isoforms produced by alternative splicing. Additional isoforms seem to exist. Mutations in TrkC have been associated with medulloblastomas, secretory breast carcinomas and other cancers.
- Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116158
- Regulatory Status For research use only.
