Left: Analysis of UCHL1 expression in cortical neuron-glial cell culture from E20 rat by Immunocytochemistry with chicken antibody to UCHL1 (1:500, red). Cells were co-stained with a mouse antibody to vimentin (green). Blue: DAPI nuclear stain. The UCHL1 antibody produces strong staining of the cell body and dendrites in neurons. The vimentin antibody stains intermediate filaments in fibroblastic and developing glial cells. Right: Western blot analysis of tissue and cell lysates using chicken antibody to UCHL1 (1:2,000, green), and a mouse anti-actin antibody (red, lanes 2-7).  protein standard,  rat brain,  mouse brain,  NIH-3T3,  HEK293,  HeLa and  SH-SY5Y cells. The single band at 24 kDa mark corresponds to UCHL1 protein which is detectable in CNS extracts and lysates of cells with neuronal properties, but not in lysates of HeLa, NIH-3T3 and other non-neuronal cells. Actin is detected with apparent molecular weight of 42 kDa and provides an excellent loading control.