Urokinase plasminogen activator surface receptor (U-PAR), Human, ELISA assay

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Catalog Number
BEK-2104

    Product Info

  • Product Name Urokinase plasminogen activator surface receptor (U-PAR), Human, ELISA assay
  • Product Description google The human Urokinase Plasminogen Activator Surface receptor (uPAR) Kit is a sandwich ELISA. The capture antibody is a polyclonal human uPAR antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known uPAR concentration are added to these wells and allowed to complex with the bound uPAR antibody. A biotinylated human uPAR polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of uPAR present in the samples. The purpose of this kit is the in-vitro quantitative determination of human uPAR in samples such as culture supernatant, serum, plasma (Heparin), plasma (EDTA) and urine. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
  • Alternative Names Monocyte activation antigen Mo3; CD87; U-PAR; PLAUR;
  • Application(s) ELISA
  • Specificity Human Urokinase Plasminogen Activator Surface receptor (uPAR)
  • Species Reactivity Human
  • Immunogen Description The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids L23-R303
  • Range 62.5 pg/mL - 4,000 pg/mL
  • Sensitivity < 4 pg/mL
  • Regulatory Status For research use only.

    Specifications

  • Product Description The human Urokinase Plasminogen Activator Surface receptor (uPAR) Kit is a sandwich ELISA. The capture antibody is a polyclonal human uPAR antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known uPAR concentration are added to these wells and allowed to complex with the bound uPAR antibody. A biotinylated human uPAR polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of uPAR present in the samples. The purpose of this kit is the in-vitro quantitative determination of human uPAR in samples such as culture supernatant, serum, plasma (Heparin), plasma (EDTA) and urine. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
  • Application(s) ELISA
  • Application Details ELISA. For the quantification of Urokinase plasminogen activator surface receptor (U-PAR) in Culture Supernatant, Serum, Plasma (Heparin), Plasma (EDTA), Urine. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
  • Target Urokinase plasminogen activator surface receptor (U-PAR)
  • Specificity Human Urokinase Plasminogen Activator Surface receptor (uPAR)
  • Target Cross-Reactivity (ELISA) There is no detectable cross-reactivity with other relevant proteins.
  • Target Host Species Human
  • Species Reactivity Human
  • Immunogen Description The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids L23-R303
  • Range 62.5 pg/mL - 4,000 pg/mL
  • Sensitivity < 4 pg/mL
  • Sample Type Culture Supernatant, Plasma (EDTA), Plasma (Heparin), Serum, Urine
  • Detection Method Colorimetric
  • Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
  • Storage Instructions Store at 2-8°C
  • Storage Temperature 2-8°C
  • Batch Number Please see item label.
  • Expiration Date This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
  • Alternative Names Monocyte activation antigen Mo3; CD87; U-PAR; PLAUR;
  • Uniprot Number Q03405
  • Uniprot Number/Name Q03405 (UPAR_HUMAN)
  • Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
  • Shipping Temperature 2-8°C (on cold packs)
  • UNSPSC CODE 41116158
  • Regulatory Status For research use only.

    Citations & References