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Vascular endothelial growth factor receptor 1, soluble (sVEGFR-1), Human, ELISA assay
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Catalog Number
BEK-2162
- Product Name Vascular endothelial growth factor receptor 1, soluble (sVEGFR-1), Human, ELISA assay
- Product Description The human Vascular Endothelial Growth Factor receptor 1 (VEGFR1) Kit is a sandwich ELISA. The capture antibody is a monoclonal human VEGFR1 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known VEGFR1 concentration are added to these wells and allowed to complex with the bound VEGFR1 antibody. A biotinylated anti-human VEGFR1 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of VEGFR1 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human VEGFR1 in samples such as culture supernatant, serum and plasma (EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Alternative Names vascular permeability factor receptor; Tyrosine-protein kinase receptor FLT; Tyrosine-protein kinase FRT; Fms-like tyrosine kinase 1; VEGFR-1; FLT1;
- Application(s) ELISA
- Specificity Human Vascular Endothelial Growth Factor receptor 1 Human sVEGFR1 isoforms, possibly primate sVEGFR1 (untested), homology to mouse, rat, chicken sVEGFR1 proteins less than 80% across the immunogen used.
- Species Reactivity Human
- Immunogen Description Recombinant sVEGFR1 amino acids 27-687 (located in the ECD domain region) produced in and purified from a NSO culture system.
- Range 156 pg/mL - 10,000 pg/mL
- Sensitivity < 4 pg/mL
- Regulatory Status For research use only.
Product Info
- Product Description The human Vascular Endothelial Growth Factor receptor 1 (VEGFR1) Kit is a sandwich ELISA. The capture antibody is a monoclonal human VEGFR1 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known VEGFR1 concentration are added to these wells and allowed to complex with the bound VEGFR1 antibody. A biotinylated anti-human VEGFR1 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of VEGFR1 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human VEGFR1 in samples such as culture supernatant, serum and plasma (EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Application(s) ELISA
- Application Details ELISA. For the quantification of Vascular endothelial growth factor receptor 1, soluble (sVEGFR-1) in Culture Supernatant, Serum, Plasma (EDTA). Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
- Target Vascular endothelial growth factor receptor 1, soluble (sVEGFR-1)
- Specificity Human Vascular Endothelial Growth Factor receptor 1 Human sVEGFR1 isoforms, possibly primate sVEGFR1 (untested), homology to mouse, rat, chicken sVEGFR1 proteins less than 80% across the immunogen used.
- Target Cross-Reactivity (ELISA) Human sVEGFR1 isoforms
- Target Host Species Human
- Species Reactivity Human
- Immunogen Description Recombinant sVEGFR1 amino acids 27-687 (located in the ECD domain region) produced in and purified from a NSO culture system.
- Range 156 pg/mL - 10,000 pg/mL
- Sensitivity < 4 pg/mL
- Sample Type Culture Supernatant, Plasma (EDTA), Serum
- Detection Method Colorimetric
- Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
- Storage Instructions Store at 2-8°C
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
- Alternative Names vascular permeability factor receptor; Tyrosine-protein kinase receptor FLT; Tyrosine-protein kinase FRT; Fms-like tyrosine kinase 1; VEGFR-1; FLT1;
- Uniprot Number P17948
- Uniprot Number/Name P17948 (VGFR1_HUMAN)
- Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116158
- Regulatory Status For research use only.
Specifications
- Specific References Agbara JO et al. (2018) Serum high sensitivity C-reactive protein, soluble FMS uric acid in pregnancy induced hypertension in a tertiary health centre. Int J Curr Res. 10(8):72384-8. Application: Human serum.