A view of neonatal rat brain cultures stained with Chicken polyclonal antibody to Vimentin C-1409-50 (red) and with Rabbit polyclonal antibody to GFAP R-1374-50 (green). These two proteins are found only in non-neuronal cells so you can\'t see any neurons, except for their nuclei in blue. Maturish astrocytes have only GFAP so appear green or have a mix of both proteins, so appear yellow. Some cells only have the vimentin (immature astrocytes, microglia and fibroblasts) and so appear red.
Left: Analysis of vimentin expression in HeLa cells by Immunocytochemistry. Cells were stained with chicken antibody to vimentin (1:10,000, green), and co-stained with a mouse anti-actin antibody (red). Blue: DAPI nuclear stain. The vimentin antibody stains the intermediate filament network, while the actin antibody labels the submembranous cytoskeleton, stress fibers, and bundles of actin associated with cell adhesion sites. Right: Western blot analysis of tissue and cell lysates using chicken antibody to Vimentin (1:5,000, red, lanes 2-6).  protein standard,  rat whole brain,  HeLa,  SH-SY5Y,  HEK293,  NIH-3T3. Vimentin protein appears as single band at around 50 kDa. The blot was simultaneously probed with a mouse antibody to MAP2C/D (green).