Left: Visualization of vimentin expression in rat cortical neuron-glial cell culture by Immunofluorescent analysis. Section was stained with mouse anti-Vimentin antibody, M-2121-100, (green, 1:5,000) and costained with rabbit polyclonal antibody to GFAP, R-1374-50, (red, 1:5,000). DAPI staining of nuclear DNA (blue). Vimentin is contained in fibroblastic, microglial and developing astrocytic cells which appear green. Maruting astrocytic cells appear red or yellow as they contain varying amounts of GFAP and vimentin.Right: Analysis by western blot of whole brain tissue lysates with M-2121-100 (green, 1:5,000) with band at approx. 50kDa. Lane 1: protein standard (red); Lane 2: HEK293; Lane 3: HeLa; Lane 4: SH-SY5Y; Lane 5: COS-1; Lane 6: C6; Lane 7: rat brain.