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Tyrosine Kinase Receptor B (TrkB), Human, ELISA assay
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Catalog Number
BEK-2338
- Product Name Tyrosine Kinase Receptor B (TrkB), Human, ELISA assay
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Product Description
The Human TrkB Kit is a sandwich ELISA. The capture antibody is a TrkB antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known TrkB concentration are added to these wells and allowed to complex with the bound TrkB antibody. A biotinylated TrkB antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of TrkB present in the samples. The purpose of this kit is the in-vitro quantitative determination of human TrkB in samples such as culture supernatant and cell lysates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Alternative Names Tropomyosin-related kinase receptor; BDNF/NT-3 growth factors receptor; Neurotrophic tyrosine kinase receptor type 2; TrkB tyrosine kinase; GP145-TrkB/GP95-TrkB; Trk-B; Ntrk2+; Trk-B;
- Application(s) ELISA
- Specificity Human. Cross-reacts with mouse and rat TrkB.
- Species Reactivity Human
- Immunogen Description The standard and immunogen for this kit is recombinant TrkB produced in and purified from the NSO expression system.
- Range 0.156 - 10 ng/mL
- Sensitivity < 10 pg/mL
- Regulatory Status For research use only.
Product Info
-
Product Description
The Human TrkB Kit is a sandwich ELISA. The capture antibody is a TrkB antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known TrkB concentration are added to these wells and allowed to complex with the bound TrkB antibody. A biotinylated TrkB antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of TrkB present in the samples. The purpose of this kit is the in-vitro quantitative determination of human TrkB in samples such as culture supernatant and cell lysates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
- Application(s) ELISA
- Application Details ELISA. For the quantification of Tyrosine Kinase Receptor B (TrkB) in Culture Supernatant, Cell Lysates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
- Target Tyrosine Kinase Receptor B (TrkB)
- Specificity Human. Cross-reacts with mouse and rat TrkB.
- Target Cross-Reactivity (ELISA) Does not show cross-reactivity with other neurotrophin receptors
- Target Host Species Human
- Species Reactivity Human
- Immunogen Description The standard and immunogen for this kit is recombinant TrkB produced in and purified from the NSO expression system.
- Range 0.156 - 10 ng/mL
- Sensitivity < 10 pg/mL
- Sample Type Cell Lysates, Culture Supernatant
- Detection Method Colorimetric
- Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
- Storage Instructions Store at 2-8°C
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date 6 months after date of receipt if unopened
- Alternative Names Tropomyosin-related kinase receptor; BDNF/NT-3 growth factors receptor; Neurotrophic tyrosine kinase receptor type 2; TrkB tyrosine kinase; GP145-TrkB/GP95-TrkB; Trk-B; Ntrk2+; Trk-B;
- Uniprot Number Q16620
- Uniprot Number/Name Q16620 (NTRK2_HUMAN)
- Scientific Background TrkB is a member of the neurotrophic tyrosine receptor kinase family. It is a membrane-bound receptor and upon neurotrophin binding, it phosphorylates itself as well as MAPK pathways members. TrkB is the receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). It is Involved in the development and/or maintenance of the nervous system.
- Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116158
- Regulatory Status For research use only.
Specifications
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Specific References
Li Z et al. (2023) Blue light at night produces stress-evoked heightened aggression by enhancing brain-derived neurotrophic factor in the basolateral amygdala Neurobiol Stress. 28:100600. Application: Rat, Tissue homogenate