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LipoFluor-P1™ Ready-to-Dilute™, Polar Lipid Tracing Reagent
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US$297.00
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Catalog Number
TR-600
- Product Name LipoFluor-P1™ Ready-to-Dilute™, Polar Lipid Tracing Reagent
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Product Description
LipoFluor-P1™ is a cell-permeant stain with an affinity for polar lipids which can be used in a wide variety of live and fixed samples. LipoFluor-P1™ provides excellent and rapid lipid staining used for fluorescent microscopy applications and automated imaging. This stain is an effective tracer of lipids (e.g. cholesterol, sphingolipid and phospholipid) and intracellular localisation. This stain allows for simple and quick application and is ideal for epifluorescence, confocal and multiphoton microscopy. It can be used on live and fixed samples and is compatible with other dyes. LipoFluor-P1™ has a large Stokes Shift, is resistant to photobleaching and demonstrates low cytotoxicity.
- Application(s) IF, ICC, Live Cell IF
- Specificity Fluorescent imaging of lipid-rich compartments. LipoFluor-P1™ is an effective tracer of lipids (e.g., cholesterol, sphingolipid, and phospholipid) and intracellular localization. Cell penetration and localisation of LipoFluor-P1™ has been confirmed in a range of cell lines; including adipocytes (3T3-L1), prostate cells (PNT2, PNT1a, LNCaP, 22RV1 and DU145), cardiomyocytes (H9c2) and neuronal cells (PC-12), and tissues; including adipose tissue (sheep and Drosophila), muscle tissue (sheep cardiac and skeletal) and brain (murine).
- Species Reactivity Human, Mouse, Other Mammals, Rat
- Concentration 10mM
- Purity Description Thin layer chromatography using alumina plates and a solvent system of ethanol and water (3:1) revealed the presence of fluorescent isomers. No amount of starting material was detected.
- Regulatory Status For research use only.
Product Info
-
Product Description
LipoFluor-P1™ is a cell-permeant stain with an affinity for polar lipids which can be used in a wide variety of live and fixed samples. LipoFluor-P1™ provides excellent and rapid lipid staining used for fluorescent microscopy applications and automated imaging. This stain is an effective tracer of lipids (e.g. cholesterol, sphingolipid and phospholipid) and intracellular localisation. This stain allows for simple and quick application and is ideal for epifluorescence, confocal and multiphoton microscopy. It can be used on live and fixed samples and is compatible with other dyes. LipoFluor-P1™ has a large Stokes Shift, is resistant to photobleaching and demonstrates low cytotoxicity.
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LipoFluor-P2™ Ready-to-Dilute™, Polar Lipid and Endoplasmic Reticulum Tracing Reagent
LipoFluor-ER2™ Ready-to-Dilute™, Endoplasmic Reticulum – Blue Tracing Reagent
- Application(s) IF, ICC, Live Cell IF
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Application Details
Fluorescent imaging of lipid-rich compartments.
Sample types: live cells, fixed cells (4% paraformaldehyde), tissue (frozen only)
Epifluorescence microscopy: YES
Confocal microscopy: YES
Multiphoton imaging: YES
Infrared spectroscopy: YES
Raman spectroscopy: YES
Ex/Em: 405 nm / 550 nm.
The typical working concentration is 10µM (1:1000 dilution) in an appropriate buffer or cell culture medium. Biosensis recommends that the end user determine optimal dilutions/concentrations. - Target Polar Lipids
- Specificity Fluorescent imaging of lipid-rich compartments. LipoFluor-P1™ is an effective tracer of lipids (e.g., cholesterol, sphingolipid, and phospholipid) and intracellular localization. Cell penetration and localisation of LipoFluor-P1™ has been confirmed in a range of cell lines; including adipocytes (3T3-L1), prostate cells (PNT2, PNT1a, LNCaP, 22RV1 and DU145), cardiomyocytes (H9c2) and neuronal cells (PC-12), and tissues; including adipose tissue (sheep and Drosophila), muscle tissue (sheep cardiac and skeletal) and brain (murine).
- Target Host Species Human
- Species Reactivity Human, Mouse, Other Mammals, Rat
- Ex/Em Max Ex/Em: 405 nm / 550 nm
- Detection Method Fluorescence
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Kit Components
Materials provided:
LipoFluor™ Stain Reagent, 1000X, 4 vials - Purity Description Thin layer chromatography using alumina plates and a solvent system of ethanol and water (3:1) revealed the presence of fluorescent isomers. No amount of starting material was detected.
- Format The reagents in the LipoFluor™ RTD™ (1000X) are all supplied in a liquid format and are ready-to-dilute.
- Concentration 10mM
- Reconstitution Instructions Dilute solutions as directed in the protocol instructions.
- Storage Instructions The liquid stock solution vials can be stored up to 12 months from date of receipt at 2-8°C, properly sealed and protected from light. Working solutions should be used within 24 hours and not stored for later use.
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date If protected from light, the liquid 10 mM stock solution vials are stable for 12 months from date of receipt, at 2-8ºC. A vial should be diluted immediately prior to use and should be used within 24 hours for best results.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 60103920
- Regulatory Status For research use only.
